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KMID : 1134820140430091342
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 9 p.1342 ~ p.1348
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Lipolytic Effect of Supercritical Extraction from Pine Cone (Pinus koraiensis) in Mature 3T3-L1 Adipocytes
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Lee Min-Hee
Nam Da-Eun
Kim Ok-Kyung
Heo Seok-Hyun
Lee Jeong-Min
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Abstract
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Seeds of Korean pine cone (Pinus koraiensis) have long been consumed as an edible food in countries located in North-East Asia, On the other hand, Korean pine cone, containing various polyphenols, is discarded as a useless garbage after removing seeds. This study investigated the lipolytic effects of pine cone extract in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was measured by Oil red O staining, free glycerol release by colorimetric reaction, and expression of genes related to lipid metabolism by real-time PCR. Compared to control, pine cone extract reduced intracellular lipid accumulation by 8.8% and increased free glycerol release by 8.2% a concentration of 5 μg/mL in differentiated 3T3-L1 adipocytes. mRNA levels of fatty acid synthesis were not significantly different between control and pine cone extract, but mRNA levels of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) significantly increased by 38.7% and 94.1% at a concentration of 5 μg/mL, respectively. Thus, pine cone extract is suggested to have lipolytic effects through induction of LPL and HSL gene expression.
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KEYWORD
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pine cone, Pinus koraiensis, 3T3-L1, lipolysis
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